| 2022-VERM-69805 | |
| Researchers at Purdue University have developed a method using Raman spectroscopy to non-destructively detect molecular fingerprints of virus-like particles (VLPs). This is the first attempt of using Raman spectroscopy to continuously detect VLPs for vaccine manufacturing. Current methods for determining quantity and quality of viral particles are employed at-line or off-line. The researchers' method continuously monitors viral particles in a continuous bioprocess setup. The invention will support the advancement of viral vector-based gene therapies and vaccine production. Technology Validation: The researchers characterized attenuated human cytomegalovirus (CMV) at a concentration between 4.5 x 109 particles/mL to 2.9 x 1011 particles/mL at flow rates between 50 µL/min to 250 µL/min. For all the concentrations and flow rates, the Raman peak remains in the same position, and it increases in intensity with higher flow rate and concentration. To determine if Raman analysis is destructive to the VLPs, the researchers tested the VLPs before and after Raman analysis with Western blot and SDS-PAGE; the results showed no damage. Advantages - non-destructive analysis - versatile (potentially capable of characterizing multiple types of VLPs) Applications - continuous monitoring of quantity and quality of VLPs - vaccine production |
|
|
Apr 12, 2023
PCT-Patent
WO
(None)
(None)
Jun 29, 2022
Provisional-Patent
United States
(None)
(None)
Apr 12, 2022
Provisional-Patent
United States
(None)
(None)
|
|
|
Purdue Office of Technology Commercialization The Convergence Center 101 Foundry Drive, Suite 2500 West Lafayette, IN 47906 Phone: (765) 588-3475 Fax: (765) 463-3486 Email: otcip@prf.org |
