Pulse Picking Device for Sensitivity Improvement of Nonlinear Optical Imaging

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Researchers at Purdue University have developed a pulse-picking device to improve the sensitivity of nonlinear microscopy technologies. When compared to conventional techniques, enhancements of sensitivity of up to 1000x for pure chemical samples and 250x for biological samples were demonstrated using the same input laser power. This approach is compatible with coherent anti-Stokes Raman scattering microscopy along with multiple other modalities. This technology can be integrated into most commercial or lab-built nonlinear optical microscopes. The primary applications of this innovation are in the chemical analysis of biological samples and label-free identification of chemicals in the pharmaceutical and biochemical industries.


1000x sensitivity improvement for pure chemical samples using coherent anti-Stoke Raman scattering

250x sensitivity improvement for biological samples using coherent anti-Stoke Raman scattering

20x sensitivity improvement for two-photon excitation fluorescence and second harmonic generation microscopy

Reduction in photodamage to biological samples

Can be integrated into commercial or lab-built optical microscopes


Nonlinear optical imaging via coherent anti-Stokes Raman scattering, second harmonic generation, sum-frequency generation, and multiphoton fluorescence microscopy modalities

Pharmaceuticals (label-free chemical identification)

Biochemistry (analysis of biological samples for biofunction or disease progression)

Technology Validation:

This technology has been validated by integrating the technology into an existing laser scanning microscope and comparing the performance to conventional approaches. This technology demonstrated a signal-to-noise (SNR) improvement of over 1000 times for the coherent anti-Stokes Raman scattering.
Sep 26, 2022
Utility Patent
United States

Sep 24, 2021
United States
Purdue Office of Technology Commercialization
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Phone: (765) 588-3475
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Email: otcip@prf.org