|Researchers at Purdue University have developed a new spectroscopic technique, fluorescence-detected mid-infrared photothermal microscopy (F-PTIR), that measures chemical compositions of complex biological and pharmaceutical samples in 3D at high spatial resolution. F-PTIR sensitively characterizes highly localized absorption of mid-infrared radiation, exhibiting higher sensitivity than refractive index-based spectroscopies. As an initial proof of concept, the researchers examined a mixture of PEG and hydrated silica gel. F-PTIR effectively discriminates between particles in the mixture and provide clear identification of the composition of individual particles. Further demonstrating the technique's utility, the researchers acquired F-PTIR images of amorphous solid dispersions of the drug ritonavir in copovidone (a soluble polymer) and identified phase-separated domains with enrichment in ritonavir. F-PTIR, which can be readily integrated into existing microscopy platforms, promises to be an important addition to the chemical microscopy and drug development toolkit.
-High Spatial Resolution
-Ability to Study Amorphous Solid Dispersions
-Pharmaceutical Research and Analysis
Technology Validation: The new F-PTIR spectroscopy technique was used for vibrationally-selective chemical imaging of pharmaceutical materials.
"Fluorescence-Detected Mid-Infrared Photothermal Microscopy"
Papers with Code, Physics
Apr 12, 2022
Apr 12, 2021
Purdue Office of Technology Commercialization
The Convergence Center
101 Foundry Drive, Suite 2500
West Lafayette, IN 47906
Phone: (765) 588-3475
Fax: (765) 463-3486