Fluorescence-Detected Mid-Infrared Photothermal Microscopy

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2021-SIMP-69445
Researchers at Purdue University have developed a new spectroscopic technique, fluorescence-detected mid-infrared photothermal microscopy (F-PTIR), that measures chemical compositions of complex biological and pharmaceutical samples in 3D at high spatial resolution. F-PTIR sensitively characterizes highly localized absorption of mid-infrared radiation, exhibiting higher sensitivity than refractive index-based spectroscopies. As an initial proof of concept, the researchers examined a mixture of PEG and hydrated silica gel. F-PTIR effectively discriminates between particles in the mixture and provide clear identification of the composition of individual particles. Further demonstrating the technique's utility, the researchers acquired F-PTIR images of amorphous solid dispersions of the drug ritonavir in copovidone (a soluble polymer) and identified phase-separated domains with enrichment in ritonavir. F-PTIR, which can be readily integrated into existing microscopy platforms, promises to be an important addition to the chemical microscopy and drug development toolkit.

Advantages
-High Spatial Resolution
-Ability to Study Amorphous Solid Dispersions
-Highly Sensitive

Applications
-Spectroscopy
-Biological Studies
-Pharmaceutical Research and Analysis

Technology Validation: The new F-PTIR spectroscopy technique was used for vibrationally-selective chemical imaging of pharmaceutical materials.

Recent Publication:
"Fluorescence-Detected Mid-Infrared Photothermal Microscopy"
Papers with Code, Physics
URL: https://physics.paperswithcode.com/paper/fluorescence-detected-mid-infrared
Apr 12, 2021
Provisional-Patent
United States
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Purdue Office of Technology Commercialization
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Phone: (765) 588-3475
Fax: (765) 463-3486
Email: otcip@prf.org