High-throughput Synthesis, Screening, and Bioanalysis Automated Platform for Identifying Drugs

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Track Code	2020-COOK-69035
Categories	Chemistry and Chemical Analysis Pharmaceuticals
Keywords	Bioassay Chemistry and Chemical Analysis Mass Spectrometry Opioids Pharmaceuticals
Public Abstract	Researchers at Purdue University have developed a high-throughput automated platform for synthesizing, reaction screening & analysis of drug candidates. The drug discovery process is expensive and time-consuming, requiring years to develop a commercial drug. This can be attributed to the manual, fragmented steps in the process. Purdue researchers have developed an automated sample-handling system that synthesizes and analyzes libraries of chemical compounds and then runs biological assays on the synthetic compounds. The system can screen 6,144 reactions in one hour at ultra-small quantities ("make-to-measure"), allowing the analysis of many reaction conditions to select the optimal conditions for producing the purest product. The system conducts analysis via desorption electrospray ionization mass spectrometry (DESI-MS). The reactions occur in the flying microdroplets generated by DESI and the resulting products are either identified by MS or deposited for enzymatic/receptor assays for cytotoxicity and bioactivity. For high-interest candidate compounds, the synthesis spray process can be scaled up. Technology Validation: The researchers' system can screen 6,144 reactions in one hour and can screen cytotoxicity and bioactivity at a rate of 384 samples per hour. Advantages: -Automated -High-throughput -Fast -Identifies ideal reaction conditions -Make-to-measure Applications -Identifying and evaluating non-addictive pain medication and high-potency overdose reversal treatment -Creating databases of synthetic opioids not yet introduced onto the streets
People	Cooks, Robert Graham (Project leader) Morato Gutierrez, Nicolas Mauricio
Intellectual Property	Application Date Nov 7, 2022 Type NATL-Patent Country of Filing United States Patent Number (None) Issue Date (None) Application Date Mar 18, 2021 Type PCT-Gov. Funding Country of Filing WO Patent Number (None) Issue Date (None) Application Date Mar 18, 2021 Type NATL-Patent Country of Filing Europe Patent Number (None) Issue Date (None) Application Date May 20, 2020 Type Provisional-Gov. Funding Country of Filing United States Patent Number (None) Issue Date (None) Application Date (None) Type NATL-Patent Country of Filing China Patent Number (None) Issue Date (None)
Contact OTC	Purdue Office of Technology Commercialization The Convergence Center 101 Foundry Drive, Suite 2500 West Lafayette, IN 47906 Phone: (765) 588-3475 Fax: (765) 463-3486 Email: otcip@prf.org