High-throughput Label-free Enzymatic Bioassays using Automated DESI-MS

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2020-COOK-69031
Researchers at Purdue University have developed a new high-throughput label-free technique for evaluating enzymatic bioassays using automated Desorption Electrospray Ionization -Mass Spectrometry (DESI-MS). Researchers study enzyme reaction kinetics in the presence of substrates, inhibitors, reactivators, and ligands as a crucial aspect of drug discovery and other biological and biomedical research. Automated DESI-MS can improve the study of enzyme kinetics by its speed (0.6 s/sample), specificity and quantitative precision. The technique has been tested with the neuromuscular enzyme acetylcholinesterase and several other enzymes with improved efficiency and accuracy over high-throughput optical assays using labeled compounds.


Advantages:
-High-Throughput
-Efficient
-Reliable
-High Linearity in Results
-Fast Readout

Potential Applications:
-Pharmaceutical
-Biomedical
-Research in Chemistry and Biochemistry

Technology Validation:
Acetylcholinesterase, a common neuromuscular enzyme, has been tested with the new label-free bioassays using automated DESI-MS, and excellent efficiency and accuracy were observed.

Recent Publication:
"High-Throughput Label-Free Enzymatic Assays Using Desorption Electrospray-Ionization Mass Spectrometry"
Angewandte Chemie, a Journal of the German Chemical Society
DOI: 10.1002/anie.202009598
May 11, 2020
Provisional-Gov. Funding
United States
(None)
(None)
Purdue Office of Technology Commercialization
1801 Newman Road
West Lafayette, IN 47906

Phone: (765) 588-3475
Fax: (765) 463-3486
Email: otcip@prf.org