|Researchers at Purdue University have developed a new high-throughput label-free technique for evaluating enzymatic bioassays using automated Desorption Electrospray Ionization -Mass Spectrometry (DESI-MS). Researchers study enzyme reaction kinetics in the presence of substrates, inhibitors, reactivators, and ligands as a crucial aspect of drug discovery and other biological and biomedical research. Automated DESI-MS can improve the study of enzyme kinetics by its speed (0.6 s/sample), specificity and quantitative precision. The technique has been tested with the neuromuscular enzyme acetylcholinesterase and several other enzymes with improved efficiency and accuracy over high-throughput optical assays using labeled compounds.
-High Linearity in Results
-Research in Chemistry and Biochemistry
Acetylcholinesterase, a common neuromuscular enzyme, has been tested with the new label-free bioassays using automated DESI-MS, and excellent efficiency and accuracy were observed.
"High-Throughput Label-Free Enzymatic Assays Using Desorption Electrospray-Ionization Mass Spectrometry"
Angewandte Chemie, a Journal of the German Chemical Society
May 11, 2020
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