Activity-Based Probes with Unnatural Amino Acids to Monitor the Proteasome in Living Cells

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2020-TRAD-68937
Researchers at Purdue University have developed a set of activity-based probes which have shown improved fluorescence properties and selectivity towards the proteasome compared to other cellular proteases. They have included unnatural amino acids and have found probes which can be utilized in various applications, including monitoring the effects of small molecule stimulators of the proteasome in live cells and comparing the relative proteasome activity across different cancer cell types.

Advantages:
-Improved Proteasome Sensitivity
-Cell-Based Assay Compatibility

Potential Applications:
-High throughput assays
-Finding Proteasome Stimulators and Inhibitors
Mar 11, 2020
Provisional-Gov. Funding
United States
(None)
(None)
Purdue Office of Technology Commercialization
1801 Newman Road
West Lafayette, IN 47906

Phone: (765) 588-3475
Fax: (765) 463-3486
Email: otcip@prf.org