| 2019-CHOP-68541 | |
| Researchers at Purdue University have developed pH-dependent fluorogenic amyloid-beta reporters for the study of Alzheimer's disease (AD). Microglial phagocytosis of amyloid-beta peptides is a critical step in the regulation of brain homeostasis during the initiation and progression of AD. Unlike common methods to study this phenomenon, this technology is specific for amyloid-beta and functions in live cells. The reporter, an isoform of human amyloid-beta tagged with a pH-dependent fluorogenic moiety, fluoresces only upon phagocytosis in the acidic intracellular phagosomes. It clearly differentiates between phagocytic and non-phagocytic cells within live human and nonhuman microglial cells. This technology promises to aid in the discovery of new therapeutics for AD. Advantages: -Facilitates live cell tracking of microglial phagocytosis -Differentiates between phagocytic and non-phagocytic microglial cells Potential Applications: -Development of Alzheimer's Therapeutics Publication: Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time. doi: https://doi.org/10.1101/2020.03.29.002857 |
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Mar 27, 2020
PCT-Patent
WO
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Mar 29, 2019
Provisional-Patent
United States
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