Portable Multiplexing Bioassay for Analyte Detection

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2019-ROBI-68413
Rapid detection of biological and chemical hazards is of upmost importance to USDA, EPA, and DoD. Likewise, companies within the food and life sciences industry are constantly concerned with contaminant detection in products. The current standard for detecting biological analytes is by the CyTOF method. In this method, samples are collected, then mixed with metal conjugated antibodies, and finally loaded into a mass cytometer. Once inside the cytometer, the metal ionizes which allows the analyte-metal conjugated antibody complex to be detected. Whilst, this system is great at detecting multiple analytes, it has several disadvantages including: samples requiring time-consuming extensive preparation and the use of the cytometer, which is a large device costing $700,000. This has created an unfulfilled need for a portable system that can quickly analyze the contents of a sample.

Researchers at Purdue have developed an instrument that can rapidly detect 15 or more analytes in a sample at once. This instrument is a similar to CyTOF in that metal conjugated antibodies are used to detect analytes. In contrast, samples are loaded into an absorbent strip with no sample preparation needed. The strips are then exposed to a laser and results are available within 15 minutes of loading the sample. The major differentiator of this instrument to CyTOF is that it is 5 percent of the cost.

Advantages:
-Detect multiple analytes at once
-Rapid and portable
-Inexpensive

Potential Applications:
-Hazard detection
-Environmental sample analysis
Sep 14, 2018
Provisional-Patent
United States
(None)
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Purdue Office of Technology Commercialization
1801 Newman Road
West Lafayette, IN 47906

Phone: (765) 588-3475
Fax: (765) 463-3486
Email: otcip@prf.org