Tool to Identify Host Proteins Involved in Viral and Bacterial Pathogenesis

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2019-TAO-68397
Purdue researchers have developed a chemical probe to facilitate quantitative proteomic analysis of potential drug targets for infectious diseases, identifying interactions between infectious particles and host proteins. Pathogens rely on their host's cells to proliferate and must bind to host cellular components. The weak and often transient nature of these interactions make capturing these interactions difficult due to harsh measures available to isolate such interactions. To aid in mitigating the high false positive rate brought about by these contemporary methods, Purdue researchers have synthesized chemical probes to label bacteria or virus particles that crosslink with interacting host proteins during infection. The probe contains a modifiable isolation tag to allow for identification of host proteins through mass spectrometry. The researchers have demonstrated this technology by isolating host proteins that directly interact with Salmonella and Zika virus, which might be critical for their pathogenesis. This method could serve as a universal tool to map the entry pathway of other pathogens.

Related Publication:
Tracking Pathogen Infections by Time-Resolved Chemical Proteomics
Angewandte Chemie, Feb 2020, Vol.132(6), pp.2255-2260
DOI: 10.1002/anie.201911078


Advantages:
-Able to identify previously unknown protein interactions
-High throughput

Potential Applications:
-Drug development
-Viral and bacterial pathogenesis research
Apr 17, 2020
Utility-Gov. Funding
United States
(None)
(None)

Apr 19, 2019
Provisional-Patent
United States
(None)
(None)
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