Purification of Elastin-like Polypeptides by Organic Solvent Extraction

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2018-THOM-68157
Protein purification is being increasingly used in a variety of ways, mostly in biomedical applications. They have beneficial physicochemical properties. The current method of purification is very labor intensive. It also results in yield loss and contamination. The required temperature for this method is very high which is what causes the yield loss during the process. This current method also takes hours to complete which is very time consuming. Sense it takes so much time to complete, it is not very cost effective either. There is a need for a new method of protein purification that is more efficient overall.

Researchers at Purdue University have developed a new technology that makes protein purification more efficient. This new method is faster than the current method. This method takes only about 30 minutes compared to hours with the current method. Also, this method does not cause loss of yield during the process because it requires a much lower temperature. Another thing is this method causes much less contamination of the protein during the process. All of these things allow this new process to be much more efficient than the current method.

Advantages:
-Faster than current method
-High yield
-Creates less contamination

Potential Applications:
-Polypeptide purification
-Organic solvent extraction

Related Publications:
Ross VerHeul, Craig Sweet and David H. Thompson, Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction. Biomater. Sci., 2018, 6, 863
DOI: 10.1039/c8bm00124c
Aug 25, 2020
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Feb 25, 2019
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Feb 26, 2018
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United States
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