Rapid Method for Determining Antibiotic Susceptibility in Bacteria

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2017-CHEN-67974
Antibiotic resistant bacteria have been drastically increasing in number due to the widespread use of antibiotics, becoming a growing public threat. To combat infections it is essential to detect bacterial susceptibility to antibiotics in the early stages of infection to reduce undesirable use of antibiotics. Current methods for antibiotic susceptibility testing (AST) require 16 hours at a minimum to complete, can only analyze specific bacteria, among other limitations. Therefore, a rapid method for detecting bacteria and profiling their antibiotics response is needed for infectious disease treatment.

Researchers at Purdue University have developed a metabolic imaging method to monitor activity in live bacteria. This system incorporates a culture-free environment and can determine antibiotic susceptibility of general bacteria within nearly one cell cycle by monitoring metabolic activity at the single cell level. AST time required using this method is reduced from at least 16 hours for conventional culture based systems to around 30 minutes. AST at the single bacterium level is especially useful for nonculturable or fastidious bacteria; metabolic activity happens faster than phenotypic growth, meaning that antibiotic susceptibility can be determined before the bacteria has the chance to replicate. This system would likely be used in laboratories and hospitals to assign the correct antibiotics to fight infections.

Advantages:
-Greatly reduced testing time
-AST before replication
-Culture-free

Potential Applications:
-Laboratories
-Hospitals
Sep 10, 2018
PCT-Patent
WO
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Sep 8, 2017
Provisional-Patent
United States
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Purdue Office of Technology Commercialization
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