Rapid Method for Determining Antibiotic Susceptibility in Bacteria

Back to all technologies

Bookmark/Download	Download as PDF
Track Code	2017-CHEN-67974
Categories	Biomedical Engineering Chemical Engineering
Keywords	Antibacterial Antibiotics Bacterial Identification Biomedical Engineering Chemical Engineering Drug Resistance Medical Imaging Medical/Health Molecular Imaging
Public Abstract	Antibiotic resistant bacteria have been drastically increasing in number due to the widespread use of antibiotics, becoming a growing public threat. To combat infections it is essential to detect bacterial susceptibility to antibiotics in the early stages of infection to reduce undesirable use of antibiotics. Current methods for antibiotic susceptibility testing (AST) require 16 hours at a minimum to complete, can only analyze specific bacteria, among other limitations. Therefore, a rapid method for detecting bacteria and profiling their antibiotics response is needed for infectious disease treatment. Researchers at Purdue University have developed a metabolic imaging method to monitor activity in live bacteria. This system incorporates a culture-free environment and can determine antibiotic susceptibility of general bacteria within nearly one cell cycle by monitoring metabolic activity at the single cell level. AST time required using this method is reduced from at least 16 hours for conventional culture based systems to around 30 minutes. AST at the single bacterium level is especially useful for nonculturable or fastidious bacteria; metabolic activity happens faster than phenotypic growth, meaning that antibiotic susceptibility can be determined before the bacteria has the chance to replicate. This system would likely be used in laboratories and hospitals to assign the correct antibiotics to fight infections. Advantages: -Greatly reduced testing time -AST before replication -Culture-free Potential Applications: -Laboratories -Hospitals
People	Cheng, Ji-xin (Project leader) Hong, Weili Seleem, Mohamed
Intellectual Property	Application Date Mar 10, 2020 Type NATL-Patent Country of Filing Europe Patent Number (None) Issue Date (None) Application Date Mar 9, 2020 Type NATL-Patent Country of Filing China Patent Number (None) Issue Date (None) Application Date Mar 9, 2020 Type NATL-Patent Country of Filing United States Patent Number (None) Issue Date (None) Application Date Sep 10, 2018 Type PCT-Patent Country of Filing WO Patent Number (None) Issue Date (None) Application Date Sep 8, 2017 Type Provisional-Patent Country of Filing United States Patent Number (None) Issue Date (None) Application Date (None) Type NATL-Patent Country of Filing Japan Patent Number (None) Issue Date (None)
Contact OTC	Purdue Office of Technology Commercialization The Convergence Center 101 Foundry Drive, Suite 2500 West Lafayette, IN 47906 Phone: (765) 588-3475 Fax: (765) 463-3486 Email: otcip@prf.org