Substrate for Protein Electron Microscopy with Reduced Non-Specific Binding

Back to all technologies
Download as PDF
Transmission electron microscopy (TEM) is a technique where electrons are transmitted through an ultra-thin specimen in order to form an image created by interactions between the electrons and specimen. CryoEM is an application of TEM that produces high resolution images of proteins. CryoEM uses grids to capture target proteins. Despite the advances of this method, cryoEM currently requires large samples and time-consuming sample preparation.

Researchers at Purdue University have developed a TEM grid for cryoEM with a coating that enables rapid and efficient capture of target proteins. The coatings limit non-specific adsorption and allow specific immobilization and random orientation of the protein target on the TEM grid. Capture of the target proteins in this manner controls their areal densities and efficiently limits film degradation. These TEM grids use smaller samples and accelerate sample preparation in comparison to available grids. These concepts can be extended to other coatings and films for TEM grids.

-Accelerated sample preparation
-Smaller samples
-Binding in multiple orientations

Potential Applications:
-Particle reconstruction analysis
-Further research and development
-High resolution cryoEM
-Structural biology
Jun 15, 2018
United States
Apr 27, 2021

Oct 21, 2020
United States

Dec 16, 2016

Aug 29, 2016
United States

Dec 17, 2015
United States
Purdue Office of Technology Commercialization
The Convergence Center
101 Foundry Drive, Suite 2500
West Lafayette, IN 47906

Phone: (765) 588-3475
Fax: (765) 463-3486