Colony Imaging and Analysis

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2015-ROBI-66977
Currently, for the identification of bacterial colonies, techniques have been developed using laser light scatter, which creates patterns or fingerprints that can be classified and used to identify the microorganisms. However, very small colonies are hard to identify and some colonies are not organized in a structured fashion, like bacteria; therefore, there is a need to have enhanced imaging other than just a laser scatter.

To solve this issue, researchers at Purdue University have developed a technique that introduces an additional colony image by recording the entire colony image and associating it with a laser scatter pattern, which is later saved in a database where features can be extracted. This method of combining regular images of the entire macroscopic area of interest with the laser scatter fingerprint allows for the evaluation of very small colonies (less than 150 microns) or for cell systems other than microbial colonies. This technology is used in conjunction with current laser scatter techniques, adding only one second per colony to the current method. Different colored light sources can be used to collect reflected or back-scattered light from which additional information can be deduced regarding the colonies. Using the image camera, non-microbial populations, such as stem cells, fibroblast colonies, and other cell colonies, can be segmented and the image processed to identify appropriate or reduced to phenotypically useful groups.

Advantages:
-Additional images used
-Enhanced imaging of colonies
-Non-microbial species identified

Potential Applications:
-Medical/Health
-Diagnostics
-Drug development
-Clinical trials
-Food safety
Oct 18, 2017
NATL-Patent
European Patent
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Oct 9, 2017
NATL-Patent
United States
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Apr 21, 2016
PCT-Patent
WO
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Apr 21, 2015
Provisional-Patent
United States
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Apr 21, 2015
Provisional-Patent
United States
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