Cell Sorting and HPV Diagnosis

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The population-wide utilization of screening cervical cytology (pap tests) has been associated with a dramatic decrease in morbidity and mortality from cervical cancer in the United States and in other industrialized nations. Despite this success, the cytological diagnosis of cervical lesions is plagued by the persistent problem of low specificity for clinically significant, high-grade lesions in patients with low-grade cytological abnormalities.

Researchers at Purdue University have developed a two part integrated assay that combines flow cytometry and multiplexed HPV genotype identification. Protein biomarker phenotypes and the presence of specific HPV genotypes are assessed for the same cell population within a clinical sample. This assay improves the overall accuracy and specificity of detection and characterization of incipient cervical disease. The assay is compatible with samples conventionally fixed with PreservCyt (Cytyc) and can be applied to residual cells initially collected as part of a normal cervical lavage. Cells that test positive for over-expression of multiple protein biomarkers are checked for high-risk HPV genotypes. If a high-risk HPV genotype is found, then detection can be done to determine the individual HPV type.

Advantages:
-Accurate diagnosis can be obtained from a small population of cells
-Directly links the presence of cancer-indicative proteins with presence of HPV
-Higher information content and more objective than visual pap tests

Potential Applications:
-Medical/Health
-Diagnostics
Nov 21, 2013
CON-Patent
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Sep 4, 2012
NATL-Patent
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Mar 4, 2011
PCT-Patent
WO
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Mar 4, 2011
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Mar 4, 2011
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Japan
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Mar 4, 2010
Provisional-Patent
United States
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Mar 10, 2009
Provisional-Patent
United States
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Purdue Office of Technology Commercialization
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