|Precision quantification of metabolite concentrations is important to identify relationships within biological systems. Traditional ways to quantify metabolites include gas and liquid chromatography for separation and mass spectrometry for identification and quantification. A major limitation to precision quantification is ion suppression.
Researchers at Purdue University have dramatically improved precision by developing a novel post-biosynthetic encoding concept using derived samples as internal standards. This cost-effective global derivation approach enables absolute quantification of known compounds and relative quantification of unknown compounds in most cases using a single LC-MS run.
-Aniline based derivation agent labels most of the metabolites or other small molecules
-Absolute quantification of known compounds
-Relative quantification of unknown compounds, no standards are necessary
-Most of these compounds can be analyzed in single LC-MS run
Apr 13, 2011
Jan 14, 2014
May 1, 2009
May 2, 2008
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