Group Specific Internal Standard Technology and Labeling Strategy

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Precision quantification of metabolite concentrations is important to identify relationships within biological systems. Traditional ways to quantify metabolites include gas and liquid chromatography for separation and mass spectrometry for identification and quantification. A major limitation to precision quantification is ion suppression.

Researchers at Purdue University have dramatically improved precision by developing a novel post-biosynthetic encoding concept using derived samples as internal standards. This cost-effective global derivation approach enables absolute quantification of known compounds and relative quantification of unknown compounds in most cases using a single LC-MS run.

Advantages:
-Aniline based derivation agent labels most of the metabolites or other small molecules
-Absolute quantification of known compounds
-Relative quantification of unknown compounds, no standards are necessary
-Most of these compounds can be analyzed in single LC-MS run
Apr 13, 2011
Utility Patent
United States
8,628,977
Jan 14, 2014

May 1, 2009
PCT-Patent
WO
(None)
(None)

May 2, 2008
Provisional-Patent
United States
(None)
(None)
Purdue Office of Technology Commercialization
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