Enhanced Protein Sensing Technologies

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Protein detection and sensing is currently done with chromatographic, electrophoretic, and mass spectroscopic technologies. Unfortunately, these techniques are expensive, inefficient, and are difficult to use for detecting important parameters, such as protein phosphorylation, glycoprotein branching, conformation, and drug binding.

Purdue University researchers have developed a novel technique for multiplexed protein analysis with enhanced sensitivity and a lower recurring chemical cost when compared to current proteomic detection and labeling technologies. This technology combines signal enhancement, optical detection, and data processing techniques to produce a new family of optical sensors. They can be used for proteomic detection, screening, analysis, and drug discovery. The proposed sensors will facilitate the measurement of changes in protein structure and composition, which would be difficult to detect using chromatographic, electrophoretic, or even mass spectroscopic proteomic sensing methods alone. This technology can be used as a highly sensitive, stand-alone protein sensor down to a single protein level capable of identifying chemical and structural information to detect protein post-translation modifications, conformational changes, and binding events. Alternatively, this technology could be integrated into chromatographic, electrophoretic, and mass spectroscopic protein separation and storage instrument, which would provide enhanced protein detection, identification, and modification sensing functionality.

Advantages:
-High throughput capabilities
-Enhanced sensitivity to detect
-Stand-alone integrated system

Potential Applications
-Medical/Healthcare
-Pharmaceuticals
-Medical Devices
-Drug Screening
Apr 8, 2005
United States
7,298,474
Nov 20, 2007

Oct 24, 2003
PCT-Patent
WO
(None)
(None)

Oct 24, 2002
Provisional-Patent
United States
(None)
(None)
Purdue Office of Technology Commercialization
1801 Newman Road
West Lafayette, IN 47906

Phone: (765) 588-3475
Fax: (765) 463-3486
Email: otcip@prf.org