High Throughput Quantitative Proteomics

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Ion/ion reaction technologies that are used to manipulate ion charge state distributions in mass spectrometers have been limited to product ion distributions that are determined solely by the kinetics of reaction. This limitation of product ion distributions results in the inability to accumulate ions in an arbitrary charge state while ions are undergoing ion/ion reactions.

Researchers at Purdue University have developed methods that allow for the mutual presence of positively charged and negatively charged ions such that the ions can react with one another and whereby the kinetics of reaction of ions of selected mass-to-charge ratios can be reduced relative to those of non-selected ions. This capability allows for highly charged ions derived from a particular high mass molecule, usually formed via electrospray ionization, to be accumulated into a selected charge state. Furthermore, this capability allows for ions present in a complex mixture of ions of like polarity to be selectively removed from the population of ions undergoing ion/ion reactions. This capability is particularly useful for the analysis of complex mixtures of high mass molecules. Such an overall capability would be attractive, for example, in proteomics applications.

Advantages:
-Provides means for accumulating ions into predetermined charge states for isolating species from complex mixtures of ions
-Isolates one or more ions of defined mass-to-charge ratio(s) and minimizes the extent to which the isolated ions undergo ion/ion reactions in the presence of ions of opposite polarity

Potential Applications:
-Mass Spectroscopy
-Chemical Analysis
-Analytical Methods
Sep 27, 2002
NATL-Patent
Canada
2,461,587
Apr 26, 2011

Nov 3, 2008
CON-Patent
United States
7,901,943
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Sep 27, 2002
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Australia
2002341862
Jan 15, 2009

Sep 27, 2002
Utility Patent
United States
7,449,170
Nov 11, 2008

Sep 24, 2008
DIV-Patent
Australia
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Sep 27, 2002
PCT-Patent
WO
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Sep 27, 2002
NATL-Patent
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Sep 27, 2001
Provisional-Patent
United States
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Purdue Office of Technology Commercialization
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