Overexpression of Histone Increases Plant Transformation by Agrobacterium

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Agrobacterium has proven to be a useful tool in the transformation of some, but not all, plants. One problem among recalcitrant plants is the failure of the T-DNA to become integrated into the host genome. As a result, transformation occurs, but it is transient.

Purdue University researchers have found that a particular histone H2A variant of Arabidopsis is involved in Agrobacterium T-DNA integration into the Arabidopsis genome. A plant carrying a mutation of this gene cannot integrate T-DNA; however, the wild-type allele can complement this mutation. In addition, overexpression of the histone H2A gene in wild-type Arabidopsis increases the efficiency of Agrobacterium mediated transformation. Because the histone H2A gene can reside on the incoming T-DNA and be expressed in the recipient cell, researchers speculate that this effect will improve transformation rates in recalcitrant plants.

Advantages:
-Stable Agrobacterium transformation of recalcitrant plants
-Unique solution to plant transformation problems

Potential Applications:
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